Human EPOR/Erythropoietin Receptor (Fc Tag) recombinant protein

Human EPOR/Erythropoietin Receptor (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type â…  single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.

Erythropoietin receptor

EPO RECEPTOR; EPO-R; ERYTHROPOIETIN RECEPTOR; EPO R; EPOR

P19235

EPOR

Human Cells

A DNA sequence encoding the extracellular domain (Met-Pro 250) of human erythropoietin receptor (NP_000112.1) precursor was expressed with the C-terminal fused Fc region of human IgG1.

Human

The recombinant human EPOR/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 460 amino acids and has a predicted molecular mass of 51.0 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEPOR/Fc monomer is approximately 55-60 kDa due to glycosylation.

> 90 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized CD131 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human EPOR with a linear range of 0.16-4 μg/ml.
2. Measured by its ability to inhibit EPOdependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is typically 4-16 ng/mL in the presence of 0.1 U/mL Recombinant Human EPO.