Mouse EPOR/Erythropoietin Receptor (His Tag) recombinant protein

Mouse EPOR/Erythropoietin Receptor (His Tag) recombinant protein

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• Material Safety Data Sheets (MSDS)

Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.

Erythropoietin receptor

ERYTHROPOIETIN RECEPTOR; EPO-R; EPO RECEPTOR; EPOR; EPO R

Q3UTV9

Epor

Human Cells

A DNA sequence encoding the extracellular domain of mouse EPOR (NP_034279.3) (Met 1-Pro 249) was expressed, with a C-terminal polyhistidine tag.

Mouse

The recombinant mouse EPOR comprises 236 amino acids with a predicted molecular mass of 26.2 kDa. As a result of glycosylation, the apparent mplecular mass of rmEPOR is approximately 30-35 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. Measured by its ability to inhibit EPOdependent proliferation of TF-1 human erythroleukemic cells.
The ED₅₀ for this effect is typically 0.1-0.5 μg/mL in the presence of 16 ng/mL Recombinant mouse EPO.
2. Measured by its binding ability in a functional ELISA.
3. Immobilized mouse EPOR-His at 10μg/mL (100μL/well) can bind biotinylated mouse EPO-His (Cat:504106).
The EC₅₀ of biotinylated mouse EPO-His (Cat:504106) is 34.5-80.6ng/mL.