Mouse CD27 (His Tag) recombinant protein

Mouse CD27 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has important role in generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.

CD27 antigen

S152; S152.LPFS2; TNFRSF7; TP55; MGC20393; T14; CD27

Q3U4X0

Cd27; Tnfrsf7

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Arg 182) of mouse CD27 (NP_001028298.1) was fused with a polyhistidine tag at the C-terminus.

Mouse

The recombinant mouse CD27 consists of 173 amino acids after removal of the signal peptide and has a predicted molecular mass of 19.6 kDa. In SDS-PAGE under reducing conditions, rmCD27 migrates as an approximately 35 kDa band due to glycosylation.

> 96 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD27 at 2 μg/ml (100 μl/well) can bind human CD70 with a linear range of 78-1250 pg/ml.