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Product Name
Mouse CD27 (His & Fc Tag) recombinant protein
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Description
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
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Protein name
CD27 antigen
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Protein short names
S152; S152.LPFS2; TNFRSF7; T14; MGC20393; TP55; CD27
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Uniprot ID
Q3U4X0
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Gene Name
Cd27; Tnfrsf7
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Arg 182) of mouse CD27 (NP_001028298.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse CD27/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 410 amino acids and has a predicted molecular mass of 46.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD27/Fc monomer is approximately 60-65 kDa due to glycosylation.
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Purity
> 94 % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD27 at 2 μg/ml (100 μl/well) can bind biotinylated human CD70 with a linear range of 0.39-12.5 ng/ml.
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Validations
Mouse CD27 / TNFRSF7 Protein (His & Fc Tag) SDS-PAGE
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