Human IL4 recombinant protein (Native)

Human IL4 recombinant protein (Native)

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as of other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibites in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and of the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy

Interleukin-4

BCGF-1,BCGF1,BSF-1,BSF1,IL-4,Interleukin-4

P05112-1

Interleukin-4; IL-4; B-cell stimulatory factor 1; BSF-1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra

E. coli

A DNA sequence encoding the mature form of human IL4 isoform 1 (P05112-1) (His25-Ser153) was expressed and purified, with an initial Met.

Human

The recombinant human IL4 consisting of 130 amino acids and migrates with an apparent molecular mass of 15 kDa as predicted.

> 95 % as determined by SDS-PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.05-0.2 ng/mL.
The specific activity of Recombinant Human IL-4 is approximately 3.2x10E4 IU/μg.