Mouse IL-4/IL4 / Interleukin-4 recombinant protein

Mouse IL-4/IL4 / Interleukin-4 recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin-4, also known as IL4, is a secreted protein which belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as of other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibites in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and of the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.

Interleukin-4

BSF1; BCGF-1; IL4; IL-4; BCGF1; MGC79402; BSF-1; RP23-188H3.4

P07750

Il4; Il-4

E. coli

A DNA sequence encoding the mouse IL4 (NP_067258.1) (His23-Ser140) was expressed with a N-terminal Met.

Mouse

he recombinant mouse IL4 consists of 119 amino acids and predicts a molecular mass of 13.4 KDa. It migrates as an approximately 14 KDa band in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

Measured in a cell proliferation assay using HT-2 mouse T cells.
The ED₅₀ for this effect is typically 0.4-2 ng/mL.