Mouse IL-4/IL4 / Interleukin-4 (Q136D, Y139D, His Tag) recombinant protein

Mouse IL-4/IL4 / Interleukin-4 (Q136D, Y139D, His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.

Interleukin-4

BCGF-1; BSF1; BSF-1; RP23-188H3.4; MGC79402; BCGF1; IL-4; IL4

P07750

Il4; Il-4

Baculovirus-Insect Cells

A DNA sequence encoding the mouse IL4 (P07750) (Met 1-Ser140, Q136D, Y139D) was expressed with a polyhistidine tag at the C-terminus.

Mouse

The recombinant mouse IL4 consists of 131 amino acids and predicts a molecular mass of 14.9 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL4-His (Cat:502101) at 10 μg/mL (100 μl/well) can bind rat IL4R-Fc (Cat:502616), The EC₅₀ of rat IL4R-Fc (Cat:502616) is 0.12-0.29 μg/mL.
2. Measured by its ability to inhibit mIL4 dependent proliferation of HT-2 cells. The ED₅₀ for this effect is typically 2-10 ng/ml in the presence of 2 ng/ml Recombinant Mouse IL4.