Human DDR2/CD167b (aa 422-855, His & GST Tag) recombinant protein

Cat.#: 504299

Size:

Special Price 442.8 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    Human DDR2/CD167b (aa 422-855, His & GST Tag) recombinant protein
  • Documents
  • Description
    Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.
  • Protein short names
    CD167B; DDR2; MIG20A; TKT; AW495251; RP11-572K18.1; NTRKR3; TYRO10
  • Uniprot ID
    Q16832
  • Source/Expression Host
    Baculovirus-Insect Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the human DDR2 (Q16832) (Arg422-Glu855) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
  • Protein Species
    Human
  • Molecular weight
    The recombinant human DDR2 /GST chimera consists of 671 amino acids and has a calculated molecular mass of 77.1 kDa. The recombinant protein migrates as an approximately 77 kDa band in SDS-PAGE under reducing conditions.
  • Purity
    > 95 % as determined by SDS-PAGE
  • Activity
    The specific activity was determined to be 8 nmol/min/mg using synthetic AXLtide peptide(CKKSRGDYMTMQIG) as substrate.
  • Validations

    Human DDR2 Kinase / CD167b Protein (aa 422-855, His & GST Tag) SDS-PAGE

    Human DDR2 Kinase / CD167b Protein (aa 422-855, His & GST Tag) SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"