Human CD80/B7-1 (His & Fc Tag) recombinant protein

Human CD80/B7-1 (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

CD80 antigen (CD28 antigen ligand 1, B7-1 antigen), isoform CRA_a

B7; TSA1; B71; CD28LG1; CD28LG; B7.1; B7-1; LAB7; LY-53; MIC17; LY53; BB1; CD80; CD28L

P33681

CD80; hCG_1780136

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Asn 242) of human B7-1 (NP_005182.1) precursor was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus.

Human

The recombinant human B7-1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 455 amino acids and predicts a molecular mass of 51.7 kDa. By SDS-PAGE, the apparent molecular mass of rh B7-1/Fc is approximately 80-90 kDa due to the glycosylation.

> 95 % as determined by SDS-PAGE

1. Measured by its ability to bind human CD28 in a functional ELISA.
2. Measured by its ability to induce IL2 secretion by Jurkat human acute T cell leukemia cells.
The ED₅₀ for this effect is 0.2-0.5μg/mL