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Product Name
FADD antibody
- Documents
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Description
FADD Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue. Positive IF detected in HT-1080 cells. Positive IP detected in A549 cells. Positive WB detected in HT-1080 cells, mouse pancreas tissue. Observed molecular weight by Western-blot: 23 kDa
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Tested applications
ELISA, IHC, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FADD antibody; GIG3 antibody; MORT1 antibody; Protein FADD antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of FADD recombinant protein (Accession Number: NM_003824). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:110447(FADD antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:110447(FADD antibody) at dilution of 1:50 (under 40x lens)
HT-1080 cells were subjected to SDS PAGE followed by western blot with Catalog No:110447(FADD Antibody) at dilution of 1:300
IP Result of anti-FADD (IP:Catalog No:110447, 4ug; Detection:Catalog No:110447 1:300) with A549 cells lysate 3040ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HT-1080 cells using Catalog No:110447(FADD Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"