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Product Name
Cynomolgus CD32b/Fc gamma RIIB/FCGR2B (His Tag) recombinant protein
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Protein name
Fc gamma receptor IIb
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Protein short names
FC[G]RII; AI528646; FCR-2; FCR-3; LYM-1; CD32; FCGRII; LY-M20; RP11-474I16.2; F630109E10RIK; FCGR2; LY-17; FCGR2A; FCRII; CD32B; FCG2; IGFR2
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Uniprot ID
Q8SPW3
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD32b (AAL92097.1) extracellular domain (Met 1-Pro 217) was fused with a polyhistidine tag at the C-terminus.
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Protein Species
Cynomolgus
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Molecular weight
The recombinant cynomolgus CD32b consists of 184 amino acids and has a calculated molecular mass of 20.9 kDa. The apparent molecular mass of the recombinant protein is approximately 28-33 kDa in SDS-PAGE under reducing conditions due to glycosylation.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA.
Immobilized cynoCD32B-His (Cat:504007) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.17-0.41 μg/ml. -
Validations
Cynomolgus CD32b / FCGR2B Protein (His Tag) SDS-PAGE
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