Mouse CD32/Fc gamma RII/FCGR2 (His Tag) recombinant protein

Mouse CD32/Fc gamma RII/FCGR2 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Receptors for Fc portion of IgG (Fcγ Rs) are members of the Ig superfamily, and are divided into three classes designated Fcγ RI (CD64), Fcγ RII (CD32), and Fcγ RIII (CD16). CD32 protein is a low affinity receptor for IgG that binds only IgG immune complexes and is expressed on a diverse range of cells such as monocytes, macrophages, neutrophils, eosinophils, platelets, and B cells. Human CD32 class is encoded by three closely related genes, and designated Fcγ RII A, B, and C which share 94-99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. CD32 is involved in a number of immune responses including antibody-dependent cell-mediated cytotoxicity, clearance of immune complexes, release of inflammatory mediators, and regulation of antibody production.

Low affinity immunoglobulin gamma Fc region receptor II

LY-M20; FCGRII; CD32; LYM-1; CD32B; CD32A; FCGR; IGFR2; FCG2; RII; LY-17; FCGR2A; CDW32; FCGR2; FCΓ FCGR2B

A0A0B4J1E6

Fcgr2b

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Arg 217) of mouse FCGR2B (NP_001070657.1) precursor was expressed with a polyhistidine tag at the C-terminus.

Mouse

The secreted recombinant mouse FCGR2B consists of 189 amino acids and has a predicted molecular mass of 21.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FCGR2B is approximately 35-40 kDa due to glycosylation.

> 98 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized mouse FCGR2B-His (CD32) (Cat:501349) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC₅₀ of biotinylated human IgG1 is 0.13-0.29 μg/ml.