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Product Name
Anti-CD32b/Fc gamma RIIB/FCGR2B antibody
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Description
Rabbit monoclonal to CD32b/Fc gamma RIIB/FCGR2B
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Tested applications
ELISA, WB, IHC-P, FCM
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Species reactivity
Human CD32b / FCGR2B
Has cross-reactivity in ELISA with Human CD64; Human CD16b
No cross-reactivity in ELISA with Human CD32a; Human CD16a; Mouse FCGR2B - Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD32b extracellular domain (rh CD32b; Ala 46-Pro 217; NP_001002274.1).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/ml
IHC-P: 0.5-5 μg/mL
FCM: 0.5-2 μg/Test
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Validations
CD32b / FCGR2B Antibody, Rabbit MAb, Western blot
CD32b / FCGR2B Antibody, Rabbit MAb, Flow cytometric
Flow cytometric analysis of CD32b expression on human lymphocytes. Human peripheral blood lymphocytes were stained with Human CD32b Monoclonal Ab (10259-R110, 1 μg/test), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.
CD32b / FCGR2B Antibody, Rabbit MAb, Immunohistochemistry
Immunohistochemical staining of formalin fixed, paraffin-embedded human lymphonode showing membrane staining of B cells and some other mononuclear cell (0.5 μg/mL).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"