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Human CD32b/Fc gamma RIIB/FCGR2B (His & AVI Tag) recombinant protein

Cat.#: 503248

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Product Information

  • Product Name
    Human CD32b/Fc gamma RIIB/FCGR2B (His & AVI Tag) recombinant protein
  • Documents
  • Protein name
    Low affinity immunoglobulin gamma Fc region receptor II-b
  • Protein short names
    FCRII; LY-17; FCGR2A; F630109E10RIK; FCGR2; FCG2; IGFR2; CD32B; AI528646; FCR-2; FCR-3; LYM-1; FC[G]RII; LY-M20; CD32; FCGRII; RP11-474I16.2
  • Uniprot ID
    P31994
  • Gene Name
    FCGR2B; CD32; FCG2; IGFR2
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the extracellular domain (Ala 46-Ile 224) of mature human CD32b (NP_001002274.1) protein was fused with a signal peptide at the N-terminus and a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
  • Protein Species
    Human
  • Molecular weight
    The recombinant mature human CD32b consists of 214 amino acids after proteolytic removal of the signal peptide and predicts a molecular mass of 24 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 35 kDa protein due to glycosylation.
  • Purity
    > 95 % as determined by SDS-PAGE
  • Activity
    1. Measured by its binding ability in a functional ELISA.
    Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD32b(Cat:503484-B). The EC50 of Human CD32b(Cat:503484-B) is 1.6 - 3.7 μg/ml.
    2. Labeling ratio of biotin to protein: 0.5
  • Validations

    Human CD32b / FCGR2B Protein (His & AVI Tag), Biotinylated SDS-PAGE

    Human CD32b / FCGR2B Protein (His & AVI Tag), Biotinylated SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"