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Product Name
Human CD32b/Fc gamma RIIB/FCGR2B (His Tag) recombinant protein
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Protein name
Low affinity immunoglobulin gamma Fc region receptor II-b
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Protein short names
FCRII; LY-17; FCGR2A; F630109E10RIK; FCGR2; FCG2; IGFR2; CD32B; AI528646; FCR-2; FCR-3; LYM-1; FC[G]RII; LY-M20; CD32; FCGRII; RP11-474I16.2
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Uniprot ID
P31994
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Gene Name
FCGR2B; CD32; FCG2; IGFR2
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Ala 46-Pro 217) of mature human CD32b (NP_001002274.1) protein was expressed with a N-terminal signal peptide and a C-terminal polyhistidine tag.
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Protein Species
Human
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Molecular weight
The recombinant mature human CD32b consists of 183 amino acids after proteolytic removal of the signal peptide and predicts a molecular mass of 20.8 kDa. By SDS-PAGE under reducing conditions, it migrates as an approximately 25-30 kDa protein due to glycosylation.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD32b at 10 μg/ml (100 μl/well) can bind human IgG2 with a linear range of 0.16-6.4 μg/ml.
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Validations
Human CD32b / FCGR2B Protein (His Tag) SDS-PAGE
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