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Product Name
Cynomolgus CD32b/Fc gamma RIIB/FCGR2B (His & AVI Tag) recombinant protein
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Protein name
Fc gamma receptor IIb
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Protein short names
LY-M20; FCGRII; CD32; RP11-474I16.2; FCR-2; AI528646; FCR-3; LYM-1; FC[G]RII; FCG2; IGFR2; CD32B; FCRII; LY-17; FCGR2A; F630109E10RIK; FCGR2
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Uniprot ID
Q8SPW3
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD32b (AAL92097.1) extracellular domain (Met 1-Gly 220) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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Protein Species
Cynomolgus
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Molecular weight
The recombinant cynomolgus CD32b consists of 211 amino acids and has a calculated molecular mass of 23.5 kDa. The apparent molecular mass of the recombinant protein is approximately 34 kDa in SDS-PAGE under reducing conditions due to glycosylation.
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Purity
> 97 % as determined by SDS-PAGE
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Activity
Labeling ratio of biotin to protein: 1.1
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Validations
Cynomolgus CD32b / FCGR2B Protein (His & AVI Tag), Biotinylated SDS-PAGE
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