Human IL-6 recombinant protein

Human IL-6 recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

cDNA FLJ61423, highly similar to Interleukin-6

HGF; BSF2; IFNB2; IL-6; IL6; HSF

Q75MH2

E. coli

A DNA sequence encoding the mature form of human IL6 (NP_000591.1) (Val 30-Met 212) was expressed, with an initial Met at the N-terminus.

Human

The recombinant human IL6 consists of 184 amino acids and migrates with an apparent molecular mass of 20.3 kDa as estimated in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL-6 at 8 μg/ml (100 μl/well) can bind recombinant human IL6R with a linear range of 1.25-20.0 ng/mL.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.75-3 ng/mL. The specific activity of Recombinant Human IL-6 is approximately 0.35 × 10⁵ IU/μg.