Rat CD3e/CD3 epsilon (Fc Tag) recombinant protein

Rat CD3e/CD3 epsilon (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.

CD3 antigen, epsilon polypeptide (Predicted)

CD3E; TCRE; FLJ18683; T3E; CD3EPSILON; CD3; IMD18; CD3-EPSILON; AI504783

D4A5M2

Cd3e; Cd3e_predicted; rCG_58471

Human Cells

A DNA sequence encoding the rat Cd3e (NP_001101610.1) (Tyr24-Asp103) was expressed with the Fc region of human IgG1 at the C-terminus.

Rat

The recombinant rat Cd3e consists 318 amino acids and predicts a molecular mass of 36.1 kDa.

> 95 % as determined by SDS-PAGE.