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CD3 epsilon antibody

Cat.#: 107129

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Product Information

  • Product Name
    CD3 epsilon antibody
  • Documents
  • Description
    CD3 epsilon Mouse Monoclonal antibody. Positive IHC detected in human tonsillitis tissue. Positive FC detected in Jurkat cells. Positive WB detected in Jurkat cells. Observed molecular weight by Western-blot: 23 kDa
  • Tested applications
    ELISA, WB, FC, IHC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    CD3 epsilon antibody; CD3E antibody; CD3-epsilon antibody; T3E antibody; TCRE antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    This antibody was obtained by immunization of CD3 epsilon recombinant protein (Accession Number: NM_000733). Purification method: Caprylic acid/ammonium sulfate precipitation.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:100-1:400

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:107129(CD3E antibody) at dilution of 1:1000

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:107129(CD3E antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107129(CD3E Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107129(CD3E Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107129(CD3E Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107129(CD3E Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    1X10^6 Jurkat cells were stained with 0.2ug CD3E antibody (Catalog No:107129, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

    1X10^6 Jurkat cells were stained with 0.2ug CD3E antibody (Catalog No:107129, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.

  • Background
    CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"