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Product Name
CD3 epsilon antibody
- Documents
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Description
CD3 epsilon Rabbit Polyclonal antibody. Positive IP detected in Jurkat cells. Positive WB detected in Jurkat cells, human spleen tissue. Positive IHC detected in human tonsillitis tissue, human lymphoma tissue, human spleen tissue. Positive FC detected in Jurkat cells. Observed molecular weight by Western-blot: 23 kDa
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Tested applications
ELISA, WB, FC, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
CD3 epsilon antibody; CD3E antibody; CD3-epsilon antibody; T3E antibody; TCRE antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD3 epsilon recombinant protein (Accession Number: NM_000733). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:109020(CD3E antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109020(CD3E Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:109020(CD3E Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0
1X10^6 Jurkat cells were stained with 0.5ug CD3E antibody (Catalog No:109020, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
IP Result of anti-CD3E (IP:Catalog No:109020, 4ug; Detection:Catalog No:109020 1:1000) with Jurkat cells lysate 3600ug.
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Background
CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.
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References
- Huang J, Guo X, Fan N. RAG1/2 knockout pigs with severe combined immunodeficiency. Journal of immunology (Baltimore, Md. : 1950). 193(3):1496-503. 2014.
- Osborne DG, Piotrowski JT, Dick CJ, Zhang JS, Billadeau DD. SNX17 affects T cell activation by regulating TCR and integrin recycling. Journal of immunology (Baltimore, Md. : 1950). 194(9):4555-66. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"