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Product Name
Anti-Phospho-eIF4E (Ser209) Rabbit antibody
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Description
Phospho-eIF4E (Ser209) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000ICC:1/100-1/200IHC:1/50-1/100
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Validations
Western blot analysis of extracts from Hela cells, untreated or treated with UV, using eIF4E (Phospho-Ser209) Rabbit pAb (166701,1:500 diluted,upper) or Beta actin Mouse mAb (200068-8F10,lower).
Western blot analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using eIF4E (Phospho-Ser209) Antibody .
Immunofluorescence staining of methanol-fixed MCF cells using eIF4E (Phospho-Ser209) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Phospho-Ser209) Antibody (left) or the same antibody preincubated with blocking peptide (right).
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Background
Swiss-Prot Acc.P06730.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"