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Product Name
Anti-Phospho-eIF4E (Ser209) Rabbit antibody
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Description
Phospho-eIF4E (Ser209) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphopeptide corresponding to residues surrounding Ser209 of human eIF4E
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000-1/10000
IHC: 1/50
ICC/IF: 1/100
IP: 1/20
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Validations
Immunocytochemistry of eIF4E (Phospho-Ser209)(green) in Hela cells using eIF4E (Phospho-Ser209) Rabbit pAb at dilution 1/50, and DAPI(blue)
Western blot detection of eIF4E (Phospho-Ser209) in K562,Rat Brain,C6,3T3 cell lysates using eIF4E (Phospho-Ser209) Rabbit pAb(1:1000 diluted).Predicted band size:25kDa.Observed band size:25kDa.
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Background
Swiss-Prot Acc.P06730.Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"