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Product Name
Anti-eIF4E Rabbit antibody
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Description
eIF4E Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human eIF4E
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/5000
IHC: 1/20
FC: 1/20
IP: 1/20
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Validations
Western blot detection of eIF4E in K562,Rat Brain,C6,3T3,Hela cell lysates using eIF4E Rabbit pAb(1:1000 diluted).Predicted band size:25kDa.Observed band size:25kDa.
Immunohistochemistry of eIF4E in paraffin-embedded Human lung cancer tissue using eIF4E Rabbit pAb at dilution 1/20
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Background
Swiss-Prot Acc.P06730.Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"