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Product Name
Anti-eIF4E (8C8) Mouse antibody
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Description
eIF4E (8C8) Mouse monoclonal antibody
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Tested applications
WB
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Species reactivity
Human, Mouse, Rat
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Alternative names
AUTS19; CBP; eIF 4E; eIF 4F 25 kDa subunit; EIF 4F; eIF-4E; eIF-4F 25 kDa subunit; eIF4E; EIF4E1; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4 E; Eukaryotic translation initiation factor 4E; Eukaryotic translation initiation factor 4E like 1 antibody
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant human eif4e protein expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
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Validations
Western blot detection of eIF4E in Jurkat, Hela, MCF7, K562, C6, 3T3, PC12, HepG2, CHO-K1, Raji, Raw264.7 and Ramos cell lysates using eIF4E mouse mAb (1:2000 diluted). Predicted band size: 25KDa. Observed band size:25KDa.
Western blot detection of eIF4E in MDA-MB-468, ZR75-1, MCF7, T47D and CHO-K1 cell lysates using eIF4E mouse mAb (1:1000 diluted). Predicted band size: 25KDa. Observed band size:25KDa.
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Background
Swiss-Prot Acc.P06730.Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"