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Product Name
Anti-p44/42 MAPK (ERK1/2) Rabbit antibody
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Description
p44/42 MAPK (ERK1/2) Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, IP, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human ERK1/2
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
ICC/IF: 1/50-1/200
IP: 1/50
FC: 1/200
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Validations
Immunofluorescent analysis of Hela cells, using ERK1/2 Antibody.
Western blot analysis of extracts from K562, Jurkat and Hela cells using p44/42 MAPK (ERK1/2) Rabbit pAb at 1:1000 dilution. Predicted band size: 42/44kDa. Observed band size: 42kDa.
Western blot analysis of ERK1/2 Antibody expression in HepG2 whole cell lysates.
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Background
Swiss-Prot Acc.P27361,P28482.ERK1 p42 MAP kinase plays a critical role in the regulation of cell growth and differentiation. Activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters.ERK2 p44 MAP kinase plays a critical role in the regulation of cell growth and differentiation. Acts as an integration point for multiple biochemical signals, and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"