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ERK1/2 antibody

Cat.#: 107194

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Product Information

  • Product Name
    ERK1/2 antibody
  • Documents
  • Description
    ERK1/2 Mouse Monoclonal antibody. Positive IHC detected in human ovary tumor tissue, human cervical cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in HeLa cells, HepG2 cells, MCF-7 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 38 kDa,43 kDa
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    ERK antibody; ERK 1 antibody; ERK1 antibody; ERK1/2 antibody; ERT2 antibody; HS44KDAP antibody; HUMKER1A antibody; Insulin stimulated MAP2 kinase antibody; MAP kinase 1 antibody; MAP kinase 3 antibody; MAP kinase isoform p44 antibody; MAPK 1 antibody; MAPK 3 antibody; MAPK3 antibody; p44 ERK1 antibody; p44 MAPK antibody; P44ERK1 antibody; P44MAPK antibody; PRKM3 antibody
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was obtained by immunization of ERK1/2 recombinant protein (Accession Number: NM_002746). Purification method: Protein G purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:1000-1:10000

    IHC: 1:20-1:200

    IF: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107194(ERK1/2 Antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107194(ERK1/2 Antibody) at dilution of 1:1000

    IP Result of anti-ERK1/2 (IP:Catalog No:107194, 5ug; Detection:Catalog No:107194 1:2000) with HeLa cells lysate 2960ug.

    IP Result of anti-ERK1/2 (IP:Catalog No:107194, 5ug; Detection:Catalog No:107194 1:2000) with HeLa cells lysate 2960ug.

    Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:107194(ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0

    Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:107194(ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0

    Immunofluorescent analysis of HepG2 cells using Catalog No:107194(ERK1/2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

    Immunofluorescent analysis of HepG2 cells using Catalog No:107194(ERK1/2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

  • Background
    ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to insulin and NGF.
  • References
    • Guan X, Gao Y, Zhou J. miR-223 Regulates Adipogenic and Osteogenic Differentiation of Mesenchymal Stem Cells Through a C/EBPs/miR-223/FGFR2 Regulatory Feedback Loop. Stem cells (Dayton, Ohio). 33(5):1589-600. 2015.
    • Sun L, Xu C, Chen G. A Novel Role of OS-9 in the Maintenance of Intestinal Barrier Function from Hypoxia-induced Injury via p38-dependent Pathway. International journal of biological sciences. 11(6):664-71. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"