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Product Name
Anti-Phospho-p44/42 MAPK (ERK1/2) (Tyr204) Rabbit antibody
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Description
Phospho-p44/42 MAPK (ERK1/2) (Tyr204) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of tyrosine 204 (T-E-Y(p)-V-A) derived from Human p44/42 MAP Kinase.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000ICC:1/100-1/200IHC:1/50-1/100
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Validations
Western blot analysis of extracts from A549 cells, untreated or treated with EGF(10ng/ml,10min), using p44/42 MAP Kinase (Phospho-Tyr204) Rabbit pAb (166889,1:500 diluted,upper) and p44/42 MAPK (Erk1/2) Mouse mAb (201246,1:1000 diluted,lower).
Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using p44/42 MAP Kinase (Phospho-Tyr204) Antibody .
Immunofluorescence staining of methanol-fixed Hela cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204) Antibody (left) or the same antibody preincubated with blocking peptide #51246 (right).
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Background
Swiss-Prot Acc.P27361.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"