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Anti-Phospho-p44/42 MAPK (ERK1/2) (Tyr204) Rabbit antibody

Cat.#: 166889

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Product Information

  • Product Name
    Anti-Phospho-p44/42 MAPK (ERK1/2) (Tyr204) Rabbit antibody
  • Documents
  • Description
    Phospho-p44/42 MAPK (ERK1/2) (Tyr204) Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Peptide sequence around phosphorylation site of tyrosine 204 (T-E-Y(p)-V-A) derived from Human p44/42 MAP Kinase.
  • Clonality
    Polyclonal
  • Formulation
    Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB:1/500-1/1000ICC:1/100-1/200IHC:1/50-1/100

  • Validations

    Western blot analysis of extracts from A549 cells, untreated or treated with EGF(10ng/ml,10min), using p44/42 MAP Kinase (Phospho-Tyr204) Rabbit pAb (166889,1:500 diluted,upper) and p44/42 MAPK (Erk1/2) Mouse mAb (201246,1:1000 diluted,lower).

    Western blot analysis of extracts from A549 cells, untreated or treated with EGF(10ng/ml,10min), using p44/42 MAP Kinase (Phospho-Tyr204) Rabbit pAb (166889,1:500 diluted,upper) and p44/42 MAPK (Erk1/2) Mouse mAb (201246,1:1000 diluted,lower).

    Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using p44/42 MAP Kinase (Phospho-Tyr204) Antibody .

    Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using p44/42 MAP Kinase (Phospho-Tyr204) Antibody .

    Immunofluorescence staining of methanol-fixed Hela cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204) Antibody .

    Immunofluorescence staining of methanol-fixed Hela cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204) Antibody .

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204) Antibody (left) or the same antibody preincubated with blocking peptide #51246 (right).

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204) Antibody (left) or the same antibody preincubated with blocking peptide #51246 (right).

  • Background
    Swiss-Prot Acc.P27361.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"