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  • Anti-ERK1 Rabbit antibody
  • Anti-ERK1 Rabbit antibody

Anti-ERK1 Rabbit antibody

Cat.#: 167241

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-ERK1 Rabbit antibody
  • Documents
  • Description
    ERK1 Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, FC, IP
  • Species reactivity
    Human, Mouse
  • Alternative names
    ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide of human ERK1
  • Clonality
    Polyclonal antibody
  • Formulation
    Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
  • Storage instructions
    Store at -20°C. Stable for 12 months from date of receipt.
  • Applications

    WB: 1/1000

    IHC: 1/50

    ICC/IF: 1/100

    FC: 1/20

    IP: 1/20

  • Validations

    Immunohistochemistry of ERK1 in paraffin-embedded Human colon cancer tissue using ERK1 Rabbit pAb at dilution 1/50

    Immunohistochemistry of ERK1 in paraffin-embedded Human colon cancer tissue using ERK1 Rabbit pAb at dilution 1/50

    Western blot detection of ERK1 in K562,Hela cell lysates using ERK1 Rabbit pAb(1:1000 diluted).Predicted band size:43kDa.Observed band size:44kDa.

    Western blot detection of ERK1 in K562,Hela cell lysates using ERK1 Rabbit pAb(1:1000 diluted).Predicted band size:43kDa.Observed band size:44kDa.

  • Background
    Swiss-Prot Acc.P27361.Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"