-
Product Name
Anti-GRP78 BiP (C-term) (9E4) Mouse antibody
- Documents
-
Description
GRP78 BiP (C-term) (9E4) Mouse monoclonal antibody
-
Tested applications
WB, ICC/IF, IHC-P
-
Species reactivity
Human, Rat
-
Alternative names
78 kDa glucose regulated protein;78 kDa glucose-regulated protein;AL022860;AU019543;BIP; D2Wsu141e;D2Wsu17e;Endoplasmic reticulum lumenal Ca(2 )-binding protein grp78; Endoplasmic reticulum lumenal Ca2 binding protein grp78;FLJ26106;Glucose Regulated Pro antibody
-
Isotype
Mouse IgG1
-
Preparation
Antigen: Purified recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E.coli.
-
Clonality
Monoclonal
-
Formulation
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
-
Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
-
Applications
WB: 1/1000
;ICC: 1/50
-
Validations
Immunohistochemical analysis of paraffin-embedded Colorectal cancer using BiP/GRP78 (C-terminus) Mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-BiP/GRP78 (C-terminus) mouse mAb (dilution 1:50).
Western blot detection of BiP/GRP78 (C-terminus) in Hela,C6,Lncap and MDA-MB-468 cell lysates using BiP/GRP78 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:78KDa.
-
Background
Swiss-Prot Acc.P11021.When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"