Anti-GRP78 BiP (C-term) (9E4) Mouse antibody

Cat.#: 168132

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Product Information

  • Product Name
    Anti-GRP78 BiP (C-term) (9E4) Mouse antibody
  • Documents
  • Description
    GRP78 BiP (C-term) (9E4) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, IHC-P
  • Species reactivity
    Human, Rat
  • Alternative names
    78 kDa glucose regulated protein;78 kDa glucose-regulated protein;AL022860;AU019543;BIP; D2Wsu141e;D2Wsu17e;Endoplasmic reticulum lumenal Ca(2 )-binding protein grp78; Endoplasmic reticulum lumenal Ca2 binding protein grp78;FLJ26106;Glucose Regulated Pro antibody
  • Isotype
    IgG1
  • Preparation
    Antigen: Purified recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E.coli.
  • Clonality
    Monoclonal
  • Formulation
    Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    ;ICC: 1/50

  • Validations

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer using BiP/GRP78 (C-terminus) Mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer using BiP/GRP78 (C-terminus) Mouse mAb (1/100 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).

    Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-BiP/GRP78 (C-terminus) mouse mAb (dilution 1:50).

    Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-BiP/GRP78 (C-terminus) mouse mAb (dilution 1:50).

    Western blot detection of BiP/GRP78 (C-terminus) in Hela,C6,Lncap and MDA-MB-468 cell lysates using BiP/GRP78 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:78KDa.

    Western blot detection of BiP/GRP78 (C-terminus) in Hela,C6,Lncap and MDA-MB-468 cell lysates using BiP/GRP78 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:78KDa.

  • Background
    Swiss-Prot Acc.P11021.When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"