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Product Name
Anti-GRP78 BiP (6H7) Mouse antibody
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Description
GRP78 BiP (6H7) Mouse monoclonal antibody
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Tested applications
WB, IHC-P
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Species reactivity
Human, Rat
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Isotype
Mouse IgG1
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Preparation
Antigen: Synthetic peptide conjugated to KLH.
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Clonality
Monoclonal
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Formulation
PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1;000-2;000
IHC: 1/100-200
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Validations
Western blot detection of Bip/GRP78 in Jurkat,MCF7,SKBR-3,Hela cell lysates using Bip/GRP78 (6H7) Mouse mAb(1:2000 diluted).Predicted band size:78KDa.Observed band size:78KDa.
Immunoprecipitation analysis of Hela cell lysates using Bip/GRP78 (6H7) Mouse mAb.
Western blot analysis of 1)Hela, 2) Rat LiverTissue with GRP78/Bip Mouse mAb diluted at 1:2,000.
Immunohistochemical analysis of paraffin-embedded Human Colon Caricnoma using GRP78/BipMouse mAb diluted at 1:200.
Immunocytochemistry staining of HeLa cells using Bip/GRP78 (6H7) Mouse mAb(dilution 1:100).
Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using GRP78/BipMouse mAb diluted at 1:200.
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Background
Swiss-Prot Acc.P11021.Plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate .
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