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Product Name
Anti-GRP78 BiP (4F11) Mouse antibody
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Description
GRP78 BiP (4F11) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
78 kDa glucose regulated protein;78 kDa glucose-regulated protein;AL022860;AU019543;BIP;D2Wsu141e;D2Wsu17e;Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78;Endoplasmic reticulum lumenal Ca2+ binding protein grp78;FLJ26106;Glucose Regulated Prote antibody
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Isotype
Mouse IgG2b
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Preparation
Antigen: Purified recombinant human BiP/GRP78 protein expressed in E.coli.
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Clonality
Monoclonal
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Formulation
PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
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Validations
Immunoprecipitation of BiP/GRP78 from Hela cell extracts using BiP/GRP78 Mouse mAb.Western blot was performed using BiP/GRP78 Mouse mAb.
Western blot analysis of extracts from 3T3,A549,C6 and Hela cell lysates using BiP/GRP78 mouse mAb (1:1000 diluted).Predicted band size:72KDa.Observed band size:72KDa.
Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at -20u2103 and using BiP/GRP78 mouse mAb (dilution 1:200).
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Background
Swiss-Prot Acc.P11021.Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"