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Anti-GAPDH Rabbit antibody

Cat.#: 169070

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Special Price 1354.8 USD

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Product Information

  • Product Name
    Anti-GAPDH Rabbit antibody
  • Documents
  • Description
    GAPDH Rabbit polyclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, IP, FC
  • Species reactivity
    Human, Mouse, Rat, Monkey, Chicken, Zebrafish
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthesized peptide derived from human GAPDH
  • Clonality
    Polyclonal
  • Formulation
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/3000-1/10000

    IHC: 1/100-1/500

    ICC/IF: 1/100-1/250

    IP: 1/50

    FC: 1/50

  • Validations

    Immunofluorescent analysis of Hela cells, using GAPDH Antibody .

    Immunofluorescent analysis of Hela cells, using GAPDH Antibody .

    Immunohistochemical analysis of paraffin-embedded (1) Rat colon; (2) Mouse testis; (3) Human uterus; (4) Human kidney, using GAPDH Antibody .

    Immunohistochemical analysis of paraffin-embedded (1) Rat colon; (2) Mouse testis; (3) Human uterus; (4) Human kidney, using GAPDH Antibody .

    Western blot analysis of GAPDH expression in (1) Hela cell lysate; (2)Jurkat cell lysate; (3)Mouse kidney lysate; (4) Mouse spleen lysate; (5) RAW 264.7 cell lysate; (6) Rat brain lysate with GAPDH Antibody.

    Western blot analysis of GAPDH expression in (1) Hela cell lysate; (2)Jurkat cell lysate; (3)Mouse kidney lysate; (4) Mouse spleen lysate; (5) RAW 264.7 cell lysate; (6) Rat brain lysate with GAPDH Antibody.

  • Background
    Swiss-Prot Acc.P04406.Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively abundant expressed in almost cell types at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some pathology factors, such as hypoxia and diabetes, increased or decreased GAPDH expression in certain cell types.
  • References
    • 1.Repression of miR-135b-5p promotes metastasis of early-stage breast cancer by regulating downstream target SDCBP.2.Jumonji domain-containing 6 (JMJD6) identified as a potential therapeutic target in ovarian cancer

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"