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Product Name
Anti-GAPDH (7E4) Mouse antibody
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Description
GAPDH (7E4) Mouse monoclonal antibody
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Tested applications
WB
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Species reactivity
Human, Monkey, Rat, Mouse, Saccharomyces cerevisiae, Pichia pastoris, Hamster
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Alternative names
38 kDa BFA-dependent ADP-ribosylation substrate;aging associated gene 9 protein;Aging-associated gene 9 protein;BARS-38;cb609;EC 1.2.1.12;G3P_HUMAN;G3PD;G3PDH;GAPD;GAPDH; GAPDH;Glyceraldehyde 3 phosphate dehydrogenase;Glyceraldehyde 3 phosphate dehydrogen antibody
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Isotype
IgM
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Preparation
Antigen: Purified recombinant human GAPDH protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Ascites containing with 0.03% Proclin300 and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/5000-1/20000
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Validations
Western blot detection of GAPDH in Hela,3T3,C6,COS7,CHO-K1,Rat brain and Jurkat cell lysates using GAPDH mouse mAb (1:5000 diluted).Predicted band size:36KDa.Observed band size:36KDa.
Western blot detection of GAPDH in S.cerevisiae and P.pastoris cell lysates using GAPDH mouse mAb (1:2000 diluted).Predicted band size:37KDa.Observed band size:37KD.
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Background
Swiss-Prot Acc.P04406.Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"