GAPDH Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells, Hela cells. Positive IHC detected in human breast cancer tissue, human lung cancer tissue. Positive FC detected in HEK-293 cells. Positive IP detected in A549 cells. Positive WB detected in HeLa cells, A549 cells, HEK-293 cells, HepG2 cells, human testis tissue, Jurkat cells, K-562 cells, MDA-MB-453s cells, mouse heart tissue, mouse kidney tissue, mouse lung tissue, PC-13 cells, Raji cells, rat brain tissue. Observed molecular weight by Western-blot: 36 kDa
ELISA, IHC, WB, IP, IF, FC
Human,Mouse,Rat,Pig; other species not tested.
G3PD antibody; GAPD antibody; GAPDH antibody; OK/SW cl.12 antibody
This antibody was obtained by immunization of GAPDH recombinant protein (Accession Number: NM_001289745). Purification method: Antigen affinity purified.
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20℃. DO NOT ALIQUOT
Western blot of Hela cell with anti-GAPDH (Catalog No:117317) at various dilutions.
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117317(GAPDH antibody) at dilution of 1:5000
IP Result of anti-GAPDH (IP:Catalog No:117317, 3ug; Detection:Catalog No:117317 1:3000) with A549 cells lysate 3500ug.
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:117317(GAPDH antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using GAPDH antibody Catalog No:117317 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
1X10^6 HEK-293 cells were stained with .2ug GAPDH antibody (Catalog No:117317, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488 -Goat anti-Rabbit IgG with dilution 1:100.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, RNA binding, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocated to the nucleus post modification such as S-nitrosylation. This antibody is a rabbit polyclonal antibody raised against full-length GAPDH of human origin. It can recognize the 36kDa GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. Read more about this antibody on the blog:http://blog.ptglab.com/index.php/gapdh-antibody-success-in-silkworm/
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"