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Product Name
Anti-GAPDH Mouse mAb (HRP Conjugate)
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Description
GAPDH Mouse mAb (HRP Conjugate)
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Tested applications
WB, ELISA
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Species reactivity
Human, Mouse, Rat
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Isotype
Mouse IgG1
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Preparation
Antigen: A synthetic peptide corresponding to residues near the amino terminus of human GAPDH protein.
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Clonality
Monoclonal
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Formulation
In PBS(pH 7.4) containing with 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500—1/5000
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Validations
Western blot detection of GAPDH in Rat brain, CHO-K1 and HUVEC cell lysates using GAPDH Mouse mAb (HRP Conjugate) (1:5000 diluted).Predicted band size:36KDa.Observed band size:36KDa.
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Background
Swiss-Prot Acc.P04406.Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis, gene expression, and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"