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Anti-GAPDH Mouse mAb (HRP Conjugate)

Cat.#: 169100

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Product Information

  • Product Name
    Anti-GAPDH Mouse mAb (HRP Conjugate)
  • Documents
  • Description
    GAPDH Mouse mAb (HRP Conjugate)
  • Tested applications
    WB, ELISA
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    IgG1
  • Preparation
    Antigen: A synthetic peptide corresponding to residues near the amino terminus of human GAPDH protein.
  • Clonality
    Monoclonal
  • Formulation
    In PBS(pH 7.4) containing with 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500—1/5000

  • Validations

    Western blot detection of GAPDH in Rat brain, CHO-K1 and HUVEC cell lysates using GAPDH Mouse mAb (HRP Conjugate) (1:5000 diluted).Predicted band size:36KDa.Observed band size:36KDa.

    Western blot detection of GAPDH in Rat brain, CHO-K1 and HUVEC cell lysates using GAPDH Mouse mAb (HRP Conjugate) (1:5000 diluted).Predicted band size:36KDa.Observed band size:36KDa.

  • Background
    Swiss-Prot Acc.P04406.Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis, gene expression, and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.
  • References

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"