Rabbit polyclonal antibody to DLL4
AOS6 antibody; delta4 antibody; hdelta2 antibody
This antigen of this antibody was synthetic peptide corresponding to n terminal human dll4.
Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Fig1:; Western blot analysis of DLL4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: 293 cell lysate; Lane 2: Mouse placenta tissue lysate
Fig2:; Flow cytometric analysis of DLL4 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody ( 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
- Yoneya T.et.al.Molecular cloning of Delta-4, a new mouse and human Notch ligand.J. Biochem. 129:27-34(2001).
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