Human DLL4/Delta-like 4 (His Tag) recombinant protein

Human DLL4/Delta-like 4 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Involved in the Notch signaling pathway as Notch ligand. Activates NOTCH1 and NOTCH4. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Essential for retinal progenitor proliferation is required for suppressing rod fates in late retinal progenitors as well as for proper generation of other retinal cell types. During spinal cord neurogenesis, inhibits V2a interneuron fate.

Delta-like protein 4

DELTA 4; DLL4; DELTA4; HDELTA2; RP23-46P4.8; DELTA LIGAND 4; MGC126344

Q9NR61

DLL4; UNQ1895/PRO4341

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Pro 524) of human DLL4 (NP_061947.1) pro-protein was expressed, with a polyhistidine tag at the C-terminus.

Human

The recombinant human DLL4 consists of 509 amino acids and predicts a molecular mass of 55.7 kDa. As a result of glycosylation, the apparent molecular mass of rhDLL4 is approximately 55-70 kDa band in SDS-PAGE under reducing conditions.

> 98 % as determined by SDS-PAGE

1. Measured by its ability to bind human Notch1 in a functional ELISA.
2. Measured by the ability of the immobilized protein to enhance BMP2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is typically 2-10 µg/mL in the presence of 500 ng/mL recombinant human BMP2.