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Product Name
Anti-Cytokeratin 7 (9G9) Mouse antibody
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Description
Cytokeratin 7 (9G9) Mouse monoclonal antibody
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Tested applications
ICC/IF, WB, IHC-P, IP
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Species reactivity
Human, Mouse, Rat
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Isotype
Mouse IgG1
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Preparation
Antigen: Synthetic Peptide of CK7
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Clonality
Monoclonal
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Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
IF: 1/50-200
WB: 1/1000-2000
IHC: 1/200
IP:1/200
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Validations
1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200
IHC staining of human lung cancer tissue, diluted at 1:200.
Western blot detection of Keratin 7 in T47D and Hela cell lysates using Keratin 7 mouse mAb(dilution 1:1000).Predicted band size:51kDa.Observed band size:55kDa.
Western blot analysis of 1) Hela, 2) Mouse Kidney, 3) Mouse Brain, diluted at 1:2000.
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Background
Swiss-Prot Acc.P08729.Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"