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Product Name
Anti-Krt7 antibody
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Description
Rabbit polyclonal antibody to Krt7
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Tested applications
WB, IHC-P
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Species reactivity
Human, Mouse, Rat
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Alternative names
K7 antibody; Krt2- antibody; Krt2-7 antibody; D15Wsu77 antibody; D15Wsu77e antibody
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Isotype
IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from the middle of mouse ck7 251-350/469
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
IHC-P:1:400-800
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Validations
Fig1: Sample:; Large intestine (Mouse) Lysate at 40 ug; Large intestine(Rat) Lysate at 40 ug; Primary: Anti- Cytokeratin 7 at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 54 kD; Observed band size: 54 kD
Fig2: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;; Incubation: Anti-Cytokeratin 7 Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig3: Sample: Large intestine (Mouse) Lysate at 30 ug; Primary: Anti- Cytokeratin 7 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution; Predicted band size: 54 kD; Observed band size: 54 kD
Fig4: Sample:; Placenta (Mouse) Lysate at 30 ug; Lung (Mouse) Lysate at 30 ug; Primary: Anti-Cytokeratin 7 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution; Predicted band size: 54 kD; Observed band size: 54 kD
Fig5: Sample:; Embryo (Mouse) Lysate at 40 ug; Primary: Anti- Cytokeratin 7 at 1/1000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 54 kD; Observed band size: 54 kD
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