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Product Name
Anti-Cytokeratin 7 (4G9) Mouse antibody
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Description
Cytokeratin 7 (4G9) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human
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Alternative names
CK 7;CK-7;ck7;Cytokeratin 7;Cytokeratin-7;Cytokeratin7;D15Wsu77e;K2C7;K2C7_HUMAN;K7;Keratin 55k type ii cytoskeletal;Keratin 7;Keratin simple epithelial type 1 k7;Keratin type II cytoskeletal 7;Keratin type ii cytoskletal 7;Keratin antibody; 55K type II cytoskelet antibody
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Isotype
IgG2b
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Preparation
Antigen: Purified recombinant human Keratin 7 protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/150;Other 1/500
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Validations
Western blot detection of Keratin 7(C-terminus) in T47D,Hela and HepG2 cell lysates using Keratin 7(C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 55KDa,Observed band size: 55KDa.
Immunocytochemistry of HeLa cells using anti-Keratin 7(C-terminus) mouse mAb diluted 1:150.
Western blot detection of Keratin 7(C-terminus) in Hela cell lysates which is positive expression and MCF7 cell lysates which is negative expression using Keratin 7(C-terminus) mouse mAb (1:500 diluted).Predicted band size: 55KDa,Observed band size: 55KDa.
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Background
Swiss-Prot Acc.P08729.Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"