Mouse SIRP alpha/CD172a (His Tag) recombinant protein

Mouse SIRP alpha/CD172a (His Tag) recombinant protein

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Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (By similarity).

Tyrosine-protein phosphatase non-receptor type substrate 1

SHPS1; SIRP; PTPNS1; BIT; RP23-37B4.2; SHP-1; AI835480; SHPS-1; MFR; P84; CD172A; SIRP ALPHA; MYD-1

P97797

Sirpa; Bit; Myd1; Ptpns1; Shps1; Sirp

Human Cells

A DNA sequence encoding the extracellular domain of mouse SIRPA (BAA20376.1) (Met 1-Asn 373) was expressed, with a C-terminal polyhistidine tag.

Mouse

The secreted recombinant mouse SIRPA comprises 353 amino acids and has a calculated molecular mass of 39.4 kDa. As a result of glycosylation, the apparent molecular mass of rmSIRPA is approximately 50-70 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized mouse SIRPA-His at 10 μg/ml (100 μl/well) can bind human CD47-Fc (Cat:504230), The EC₅₀ of human CD47-Fc (Cat:504230) is 0.05-0.13 μg/ml.