Human SIRP alpha/CD172a (His Tag) recombinant protein

Human SIRP alpha/CD172a (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

Tyrosine-protein phosphatase non-receptor type substrate 1

MYD-1; P84; CD172A; SIRP ALPHA; MFR; AI835480; SHPS-1; RP23-37B4.2; SHP-1; PTPNS1; BIT; SIRP; SHPS1

P78324

SIRPA; BIT; MFR; MYD1; PTPNS1; SHPS1; SIRP

Human Cells

A DNA sequence encoding the human SIRPA (NP_542970.1) extracellular domain (Met 1-Arg 370) was expressed, fused with a polyhistidine tag at the C-terminus.

Human

The recombinant human SIRPA consists of 351 amino acids and predictes a molecular mass of 39 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhSIRPA is approximately 45-50 kDa due to glycosylation.

> 98 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized human SIRPA-His at 10 μg/ml (100 μl/well) can bind human CD47-Fc (Cat:504230), The EC₅₀ of human CD47-Fc (Cat:504230) is 10.1-23.5 ng/ml.