Ferret TNF-alpha/TNFA recombinant protein

Ferret TNF-alpha/TNFA recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

Tumor necrosis factor alpha

TNF-A; TNFA; TNFALPHA; TNF-ALPHA; TNFSF2; DIF; TNFSF1A; MGC151434; TNF; DADB-70P7.1

A3FBF1

TNF alpha; TNF; TNF-a

E. coli

A DNA sequence encoding the mature form of Ferret (Mustela putorius furo) TNF? (A3FBF1) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.

Ferret

The recombinant Ferret TNF? consists of 158 amino acids and has a calculated molecular mass of 17.3 kDa. The apparent molecular mass of the recombinant protein is approximately 16 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The EC₅₀ for this effect is typically 50-200 pg/ml.