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Anti-Tnf antibody

Cat.#: 176684

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-Tnf antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to Tnf
  • Tested applications
    WB, ELISA
  • Species reactivity
    Mouse
  • Alternative names
    DI antibody; Tn antibody; DIF antibody; TNF- antibody; Tnfa antibody; Tnfs antibody; TNF-a antibody; TNFSF2 antibody; Tnlg1f antibody; Tnfsf1a antibody; TNFalpha antibody; TNF-alpha antibody
  • Isotype
    IgG
  • Preparation
    This antigen of this antibody was synthetic peptide within human tnf alpha aa 40-80 and aa 170-210.
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:1,000

    ELISA: 1:10,000-20,000

  • Validations

    Fig1:; Western Blot analysis of recombinant murine TNF-alpha using rabbit TNF alpha antibody.; Western blot analysis of TNF alpha on recombinant protein lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

    Fig1:; Western Blot analysis of recombinant murine TNF-alpha using rabbit TNF alpha antibody.; Western blot analysis of TNF alpha on recombinant protein lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • Background
  • References
    • Aggarwal, B.B. (2003) Nat Rev Immunol 3, 745-56.
    • Hehlgans, T. and Pfeffer, K. (2005) Immunology 115, 1-20.
    • Lin, P.L. et al. (2007) J Investig Dermatol Symp Proc 12, 22-5.
    • Brennan, F.M. and McInnes, I.B. (2008) J Clin Invest 118, 3537-45.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"