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Product Name
Human TNF-alpha/TNFA recombinant protein
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Description
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
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Protein name
Tumor necrosis factor
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Protein short names
DADB-70P7.1; TNF; MGC151434; DIF; TNFSF1A; TNFSF2; TNFALPHA; TNF-ALPHA; TNF-A; TNFA
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Uniprot ID
Q5STB3
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Gene Name
TNF; TNFA; TNFSF2
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the human TNF-? soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human TNF-? consists of 158 amino acids and has a predicted molecular mass of 17.4 kDa which is also estimated by SDS-PAGE.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is typically 20-100 pg/ml, corresponding to a specific activity of 3 x 10E6-2 x 10E7 units/mg. -
Validations
Human TNF-alpha / TNFA Protein SDS-PAGE
Related Products / Services
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