Human TNF recombinant protein (Native)

Human TNF recombinant protein (Native)

• DataSheet
• Material Safety Data Sheets (MSDS)

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.

TNF-alpha Protein & AntibodyImmune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy

Tumor necrosis factor

DIF,TNF-alpha,TNFA,TNFSF2

P01375

Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a [Cleaved into: Tumor necrosis factor, membrane form; N-terminal fragment; NTF; Intracellular domain 1; ICD1; Intracellular domain 2; ICD2; C-domain 1; C-domain 2; Tumor necrosis factor, soluble form]

E. coli

A DNA sequence encoding the human TNF-α soluble form (NP_000585.2) (Val77-Leu233) was expressed with an initial Met at the N-terminus.

Human

The recombinant human TNF-α consists of 158 amino acids and has a predicted molecular mass of 17.5 kDa.

> 95 % as determined by SDS-PAGE

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The specific activity of this protein is approximately 7.6x10E4 IU/μg.