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Product Name
TFAM antibody
- Documents
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Description
TFAM Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human testis tissue. Positive WB detected in MCF7 cells, A431 cells, HEK-293 cells, HeLa cells, HepG2 cells, human testis tissue, Jurkat cells, K-562 cells. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 25 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
MtTF1 antibody; mtTFA antibody; TCF 6 antibody; TCF6 antibody; TCF6L1 antibody; TCF6L2 antibody; TCF6L3 antibody; TFAM antibody; Transcription factor 6 antibody; Transcription factor 6 like 2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TFAM recombinant protein (Accession Number: NM_003201). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:115997(TFAM antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:115997(TFAM Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:115997(TFAM Antibody) at dilution of 1:50
IP Result of anti-TFAM (IP:Catalog No:115997, 4ug; Detection:Catalog No:115997 1:800) with HEK-293 cells lysate 3200ug.
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Background
TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"