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Product Name
TFAM antibody
- Documents
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Description
TFAM Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, A431 cells, HeLa cells, HL-60 cells, Jurkat cells, MCF7 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue, human brain tissue. Observed molecular weight by Western-blot: 25 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
MtTF1 antibody; mtTFA antibody; TCF 6 antibody; TCF6 antibody; TCF6L1 antibody; TCF6L2 antibody; TCF6L3 antibody; TFAM antibody; Transcription factor 6 antibody; Transcription factor 6 like 2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of TFAM recombinant protein (Accession Number: NM_003201). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:115995(TFAM antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:115995(TFAM Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human kidney slide using Catalog No:115995(TFAM Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells using Catalog No:115995(TFAM Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"