Human HtrA2 (His Tag) recombinant protein

Human HtrA2 (His Tag) recombinant protein

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Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.

Serine protease HTRA2, mitochondrial

PRSS25; PARK13; AI481710; MND2; OMI; HTRA2

O43464

HTRA2; OMI; PRSS25

E. coli

A DNA sequence encoding the mature form of human HTRA2 (O43464-1) (Ala 134-Glu 458) was expressed, with a polyhistide tag at the C-terminus.

Human

The recombinant human HTRA2 consisting of 336 amino acids and has a calculated molecular mass of 36.5 kDa as estimated in SDS-PAGE under reducing conditions.

> 87 % as determined by SDS-PAGE

Protease activity demonstrated by HtrA2 cleavage of bovine β-casein (Sigma, Catalog # C-6905). Incubation of β-casein at 0.2 mg/mL with Recombinant Human HTRA-2 at 0.02 mg/mL (ratio of 10:1) for 60 minutes at 45℃ in 50 mM Tris, pH 8.0, which results in >95% cleavage of β-casein, as revealed by SDS-PAGE.