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Product Name
HTRA2 antibody
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Description
HTRA2 Rabbit Polyclonal antibody. Positive IP detected in Raji cells. Positive WB detected in HEK-293 cells, COS-7 cells, HeLa cells, human heart tissue, Jurkat cells, MCF7 cells, mouse skeletal muscle tissue, Raji cells. Positive IHC detected in human kidney tissue, human colon tissue, human thyroid tissue. Observed molecular weight by Western-blot: 36 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat, Monkey; other species not tested.
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Alternative names
HtrA serine peptidase 2 antibody; HTRA2 antibody; OMI antibody; PARK13 antibody; PRSS25 antibody; Serine protease 25 antibody; Serine proteinase OMI antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of HTRA2 recombinant protein (Accession Number: NM_013247). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111715(HTRA2 antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111715(HTRA2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:111715(HTRA2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-HTRA2 (IP:Catalog No:111715, 4ug; Detection:Catalog No:111715 1:500) with Raji cells lysate 2000ug.
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Background
HTRA2(High temperature requirement protein A2) is also named as OMI, PRSS25 and belongs to the peptidase S1B family. HTRA2 normally exists in the mitochondria, can cause MMP3 activation in the cytosol under a cell stress condition, which can ultimately lead to demise of dopaminergic neuronal cells(PMID: 22265821). It inhibits mitochondrial superoxide generation, stabilizes mitochondrial membrane potential, and prevents apoptosis at baseline and in response to extracellular inducers of mitochondrial stress(PMID: 18772386). This protein has 4 isoforms produced by alternative splicing.
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References
- Behbahani H, Pavlov PF, Wiehager B, Nishimura T, Winblad B, Ankarcrona M. Association of Omi/HtrA2 with γ-secretase in mitochondria. Neurochemistry international. 57(6):668-75. 2010.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"